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. 2019 Feb 13;10:217. doi: 10.3389/fimmu.2019.00217

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Copyright © 2019 Eken, Yetkin, Vural, Okus, Erdem, Azizoglu, Haliloglu, Cakir, Turkoglu, Kilic, Kara, Dönmez Altuntaş, Oukka, Kutuk, Mirza and Canatan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Human ILC subsets express S1P receptors and migrate toward S1P analogs in vitro. (A) Gating strategy for human ILC subset isolation from tonsil and cord blood. (B) Sorted ILC subsets were used to determine relative gene expression of S1P receptors (S1PR1, S1PR2, S1PR3, S1PR4, S1PR5) via real time qPCR. (C) Human tonsil ILC1 and ILC3 were stained for S1PR1 to determine protein expression, a representative histogram flow plot with isotype control. (D) Migration of ILC1 and ILC3 cultured in serum free media toward FTY720 and SEW2871 gradient. Percentage of cells migrating into lower chamber of a trans-well plate quantified. (E) Migration of ILC1 and ILC3 pretreated with serum free media, FTY720 or SEW2871 for 2 h toward FTY720. Percentage of cells migrating was charted. *p < 0.05. Experiments in (B–E) were performed three separate times.