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Fingolimod does not alter IFN-γ production by ILC1 at low doses. (A) ILC1s sorted from tonsils (gated as Lin-CD3-CD161+CD127+ckit-CRTH2-) were cultured in charcoal stripped FBS-supplemented media containing IL-12/IL-23/IL-1B/IL-7, 20 ng each, with increasing concentrations of FTY70 for 3 days IFN-γ production was measured with intracellular staining (A, a representative plot) and quantified (B) and mRNA levels were quantified via real time qPCR (C).
