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. 2019 Feb 8;13:35. doi: 10.3389/fncel.2019.00035

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Copyright © 2019 Cartier, Garcia-Olivares, Janezic, Viana, Moore, Lin, Caplan, Torres and Kim.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SUMO1 overexpression decreases DAT ubiquitination. HEK cells were transfected with DAT and ubiquitin to improve DAT-ubiquitin recovery. DAT-ubiquitin was immunoprecipitated by mouse anti-ubiquitin antibody in cells transfected with or without SUMO1-HA. Recovered DAT-ubiquitin was detected with anti-DAT (MAB) antibody. Inputs for DAT, ubiquitin, SUMO-HA are displayed. DAT was detected with anti-DAT (MAB) antibody. Di-ubiquitin is depicted with a star and it was detected with anti-ubiquitin (mouse) antibody. SUMO1-HA was detected with mouse anti-HA antibody. β-actin as a loading control is shown at the bottom. Data quantification from recovered DAT-ubiquitin represents mean ± SE and statistical significance from normalized control (*p < 0.05) was determined by a two-sided, Student’s t-test (four independent experiments).