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. Author manuscript; available in PMC: 2019 Feb 22.
Published in final edited form as: Lab Chip. 2019 Feb 12;19(4):562–573. doi: 10.1039/c8lc01108g

Fig. 4. Voronoï tessellation analysis of clusters formed in different viscosity systems.

Fig. 4

(a) Representative confocal fluorescence micrographs of myoblasts (green) acoustically patterned within PEG norbornene hydrogels containing a concentration range of high molecular weight PEG dopant (0–3% w/v). The hydrogels were photocrosslinked after 5 min of patterning, swollen overnight in PBS and then imaged. An undoped control was included without any applied field. Scale bars = 200 μm. (b) Rheological creep tests at 2 Pa stress were used to characterize the viscosity of the PEG norbornene precursor solution with a PEG dopant concentration of 0% (black), 1% (magenta), 2% (blue) and 3% (green). (c) The cluster area profile was measured for each dopant concentration to provide a quantitative measure of pattern formation across the viscosity range. Data was collected from three separate images per group, plotted as median ± interquartile range and statistically treated using a Kruskal–Wallis test with Dunn's correction for multiple comparisons (n.s. is nonsignificant, *** is p < 0.001). (d) Voronoï tessellation was also used to measure the proportion of total seeds that were detected within a cluster. Data was collected from three separate images per group and plotted as mean, interquartile range and 95% confidence intervals.