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. Author manuscript; available in PMC: 2019 Aug 15.
Published in final edited form as: Science. 2019 Jan 31;363(6428):710–714. doi: 10.1126/science.aaw5569

Figure 1. Structure of a human P complex reveals unexpected exon ligation factors.

Figure 1

(A) Overview of the human P complex spliceosome complex. EJC, Exon junction complex; NTC, Prp19-associated complex; NTR, Prp19-related complex. (B and C) Comparison of the P (present work) and C* (22) complexes reveals novel factors. Note the presence of mRNA and the docked 3´-splice site in our P-complex structure. Dashed lines indicate possible path of the intron not visible in the density. The intron is shown in gray, the 5´-exon in orange, and the 3’-exon in yellow. Prp8EN, Prp8 Endonucelase domain; Prp8N, Prp8 N-terminal domain. (D) Binding of the substrate in the active site cavity of P complex. Prp8RT, Prp8 reverse-transcriptase domain. (E) The 3´SS is recognized by the 5´SS and the BP adenosine in the human P complex. (F) 3´SS recognition in the yeast P complex (7).