Figure 2. PTPN11 is activated in human melanoma and regulates ERK phosphorylation in BRAF wt melanoma cell lines.

Mutations identified in human cancers (adapted from cBioPortal): SH2 and Y phosphatase domains; Gray: missense mutation; Black: in frame deletion (A). Western blot analysis of human metastatic melanoma specimens and WM793 cell lysates (“C”) as a control (B) and melanoma cell lines (C) with pPTPN11 (Y542), PTPN11, and β-actin antibodies. The effect of PTPN11 KD with siRNAs (A & B) on ERK activation in BRAF or NRAS mutant and BRAF/NRAS wt melanoma cells (D) and NRAS activation ((RAS-GTP pull down detected with NRAS antibody) in NRAS mutant cells (E).