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Expression and localization of SLC transporters in representative Huh7 cells. Huh7 cells were cultured for 1 or 4 weeks, followed by immunostaining of transport proteins (green) with either anti-NTCP, anti-OSTα, anti-OSTβ, anti-OATP1B or anti-OATP2B1, and Alexa Fluor® 488-labeled secondary antibodies. Human hepatocytes were cultured in sandwich configuration for 5 days followed by immunostaining. Nuclei were visualized with DAPI (blue).
