Co-localization of transporters and filamentous actin (F-actin) in representative Huh7 cells cultured for 4 weeks. Transporters (green) were detected with anti-OATP1B, anti-OATP2B1, anti-OSTα, anti-OSTβ, anti-MRP2 and anti-MRP4 together with Alexa Fluor® 488-labeled secondary antibodies. Actin filaments and nuclei were visualized with Alexa Fluor® 594-labeled phalloidin (red) and DAPI (blue), respectively.