Fig 6. Semi-quantitative RT-PCR showing the transcript levels of Pp-pme in different host plants at different time points after infection.

Total RNA extracted from nematode infected roots of economically important host plants (A) soybean, (B) potato, (C) corn, and (D) alfalfa was used to validate the relative expression of Pp-pme at different days after nematode infection (DAI). The nematode 18S rDNA gene was used as internal control to validate the presence of P. penetrans within the infected roots, while specific plant reference genes were used for each specific host plant. Wild-type (WT) correspond to non-infected plants.