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. 2019 Feb 22;14(2):e0212540. doi: 10.1371/journal.pone.0212540

Fig 7. Phenotypic changes in Nicotiana benthamiana plants infected with the recombinant PVX-Pp-pme virus.

Fig 7

All photos were taken 14 days after inoculation. (A) characteristic mosaic-like symptom developed in N. benthamiana plants infected with transcripts generated from the empty PVX vector. (B-C) Distinct leaf chlorosis symptoms (B) and lesion-like spots (C) developed in N. benthamiana plants infected with PVX-Pp-pme transcripts, respectively. (D) Stem and branches of the N. benthamiana plant infected with transcripts generated from the empty PVX vector displayed no symptoms. (E-F) Lesion-like symptoms observed on stems and branches of N. benthamiana plants infected with PVX-Pp-pme transcripts. (G) Stem and roots of N. benthamiana plant infected with empty PVX vector displayed no symptoms. (H) Browning in the area of the stem-root joint and lesion-like spots observed on the roots of N. benthamiana plants infected with PVX-Pp-pme transcripts. (I-J) Detection of PVX-WT and PVX-Pp-pme transcripts in inoculated N. benthamiana plants by semi-quantitative RT-PCR. L1, L2: leaves from two independently-inoculated plants; R1, R2: roots from two independently-inoculated plants.