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. 2019 Feb 22;14(2):e0212856. doi: 10.1371/journal.pone.0212856

Fig 5. Effect of antibiotic treatment on TrkB distribution in juvenile mouse small intestine LMMP whole-mount preparations.

Fig 5

Representative confocal microphotographs showing co-staining of TrkB (green) and HuC/D (pan-neuronal marker, red) in LMMPs obtained from CTR (A-C) and ABX-treated mice (D-F). In LMMPs obtained from both CTR and ABX-treated mice TrkB stained the soma of both large and medium neurons with an ovoidal shape (arrow). TrkB immunoreactivity was also found in discontinuous prolongations surrounding neurons and in the interconnecting fibres between ganglia (asterisk). In CTR preparations, TrkB faintly stained the soma of few neurons. After antibiotic treatment, the number of myenteric neurons staining for TrkB was significantly higher than in CTR (D-E). Bars: 50 μm. (G) Percentage of TrkB immunoreactive myenteric neurons in small intestine of CTR and ABX-treated mice. Values are given as mean ± SEM of at least 20 fields for each intestinal region. P<0.001 vs CTR animals by unpaired Student’s t test.