Fig 2. TCF7L2 binds DNA harboring disease-associated rs6651252 variant with stronger affinity.

(A). DNA sequence flanking rs6651252 (asterisk) and the adjacent TCF consensus motif (underlined). (B). Western blot analysis of TCF7L2 from HCT116 protein lysates that were incubated with the biotinylated DNA probes indicated and subsequently precipitated with streptavidin conjugated magnetic beads. A probe containing mutations in the consensus TCF binding element (mutant TBE) was used as a negative control. (C). as in (B) except the biotinylated probe harboring the ancestral T variant was used in all reactions. As indicated below, increasing concentrations of unlabeled probes containing T or C were included in the binding reactions prior to precipitation.