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. 2019 Feb 22;14(2):e0212782. doi: 10.1371/journal.pone.0212782

Fig 1. Effect of OMT in viability, apoptosis and Caspase 3 expression in H9c2 cells exposed to DTX.

Fig 1

(A) Histograms showing cell viability measured by MTT assay in H9c2 cell treated with DTX after 24 h. (B) MTT cell viability assay in H9c2 cells treated with DTX and/or OMT after 24 hours. (C) Annexin V flow cytometry assay on H9c2 cell treated with DTX and/or OMT. (D) Representative density-plots images of Annexin V-FITC/propidium iodide (PI) double staining flow cytometry. (E) Caspase-3 levels in H9c2 cells treated with DTX and/or OMT after 24 hours. (F) Representative western blot autoradiographic images. Data are expressed as mean ± SEM of three or four independent experiments. Statistical significance *, ** and ***p < 0.05, 0.01, and 0.001 vs. control; #, ##, ### p< 0.05, 0.01 and 0.001 DTX-OMT vs. DTX.