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. 2019 Feb 22;14(2):e0212782. doi: 10.1371/journal.pone.0212782

Fig 2. Effect of OMT in ROS production and NADPH oxidase (NOX4) expression in H9c2 treated with DTX.

Fig 2

(A) ROS levels determined by flow cytometry using DHE staining in H9c2 cells treated with DTX and/or OMT after 24 hours and/or pre-treated with NAC for 1h. (B) Representative histogram images of DHE staining. (C) Histograms showing NOX4 mRNA expression measured by real-time polymerase chain reaction in H9c2 cell treated with DTX after 24 h. Data represent the means ± SEM from at least three independent experiments. Statistical significance *, ** and ***p < 0.05, 0.01, and 0.001 vs. control; #, ##, ### p< 0.05, 0.01 and 0.001 DTX-OMT vs. DTX.