Fig 2. Overexpression of NAMPT elevates intracellular NAD⁺ levels and reduces TG contents in ethanol-treated primary hepatocytes.

Mouse primary hepatocytes were cultured with 100 mM ethanol for 48 hours. (A) Adenovirus-mediated NAMPT-HA expression was determined by western blot. (B) Densitometric analysis of the immunoblot data in Panel A. (C and D) Intracellular NAD⁺ (C) and TG (D) levels were assessed in primary hepatocytes infected with GFP or NAMPT adenoviruses. Data are shown as mean ± S.E.M (n = 3 for each group). *p < 0.05 for Vehicle vs EtOH.