Fig 4. NAMPT overexpression ameliorates chronic-binge ethanol-induced fatty liver injury in mice.
Mice were fed with control or ethanol diet as described in Fig 3; adenoviruses were injected into mice through tail vein at a dosage of 1x109 pfu/mouse. (A) Adenovirus-mediated NAMPT-HA expression was analyzed by western blot. (B) Densitometric analysis of the immunoblot data in Panel A. (C and D) NAD+ (C) and NADH (D) concentrations were examined in mouse liver tissues. (E and F) Hepatic and serum TG levels were assessed. (G and H) H&E staining (G) and oil-red O staining (H) of liver sections. Scale bar, 50 μm. (I and J) Serum ALT (I), AST (J) levels were analyzed. Data are shown as mean ± S.E.M (n = 5–6 for each group). *p < 0.05 for Ad-GFP vs Ad-NAMPT; #p < 0.05 vs Pair-fed Ad-GFP.