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| Protocol | Primer Name | Sequence (5→3) | Amplicon size (bp) |
|---|---|---|---|
| Trans-PCR | Trans1 | TATGTATCCACCGTAGCCAGTC | 687 |
| Trans2 | CCCAACAACACCTCCTTATTC | ||
| Nested PCR | 261F | GAGCGAACCATTGGTATCG | 203 |
| 463R | CTTTAACAGCGCTTGAACGT | ||
| Real-Time PCR | tmQ-koorts4-fw | AAAACGGATAAAAAGAGTCTGTGGTT | 70 |
| tmQ-koorts4-rv | CCACACAAGCGCGATTCAT | ||
| tmQ-koorts4-fam-tamra | 6-FAM-AAAGCACTCATTGAGCGCCGCG-TAMRA |
