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. 2019 Feb 11;15(2):e1007646. doi: 10.1371/journal.pgen.1007646

Fig 2. Putative long-range interaction at the speF locus as predicted by RNAfold.

Fig 2

The sequence starts at the upper left side of the slide and the red asterisk indicates its continuation. The Shine-Dalgarno sequences of orf34 and speF, both AUGs and orf34 stop codon are indicated in magenta. The structure predicted harbors a ring-like structure encompassing nucleotides 175 to 449 followed by a long helix in which nucleotides 99–125 of orf34 basepair with nucleotides 502–535 overlapping the ribosome binding site of speF and nucleotides 155–175 upstream of orf34 basepair with nucleotides 450 to 454 and 482 to 500 upstream of ribosome binding site of speF. Mutations that disrupt the helix and compensatory mutations that restore helix-formation are indicated by arrows (GU at position 116,117 and CA at position 510,511). Premature transcription termination site by Rho is indicated (T).