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. 2019 Feb 11;15(2):e1007646. doi: 10.1371/journal.pgen.1007646

Table 3. Translation of orf34 facilitates translation of speF.

Genotypea Translation fusion +BCM
P-orf34AAA-speF-lacZ 95 ± 33
ΔP-orf34AAA C116G;A117U-speF-lacZ 1037 ± 202
ΔP-orf34AAA U510A;G511C-speF-lacZ 309 ± 74
ΔP-orf34AAA C116G;A117U, U510A;G511C -speF-lacZ 66 ± 11

aThe orf34-speF fusions harbor the promoter region (P), orf34 and speF fused to lacZ. All fusions carry orf34AAA mutation in addition to the mutations that prevent formation of the hybrid as mentioned. BCM was added at OD600 of 0.2 for 1 hour. Results are displayed as mean of 4 colonies ± standard deviation