Fig 3. LAV replication in popliteal lymph nodes (PLN) and serum.

CD-1 mice were infected with equal genomes of the LAV candidates corresponding to 10³ pfu of WT EEEV (WT). At 24hpi, single PLN (A) or serum (B) were harvested and a plaque assay was performed. Each point represents an individual mouse. n = 6–8 mice, 2 independent experiments. Data is log₁₀ transformed and the geometric mean (bar) with geometric mean standard deviation graphed and compared between groups. Single mutant LAVs were compared to WT, double mutant LAVs were compared to single mutant LAVs, triple mutant LAVs were compared to the double mutant LAVs, and the quadruple mutant LAV was compared to the triple mutant LAVs. Only comparisons that were significant are represented, all other comparisons were non-significant. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 by one-way analysis of variance with Tukey’s multiple-comparison test of log-transformed data.