Table 1. Michaelis-Menten kinetic parameters for the initial rate of L-Tyr uptake by TgApiAT5-3a.
[L-Tyr]trans (mM) | Scatchard Linear Regression | Michaelis-Menten Plot | ||
---|---|---|---|---|
K0.5 (μM) | Vmax (pmol/min × oocyte) | K0.5 (μM) | Vmax (pmol/min × oocyte) | |
0 | 0.36 | 5.23 | 0.37 | 5.43 |
0.02 | 0.37 | 5.70 | 0.31 | 6.06 |
0.08 | 0.38 | 6.25 | 0.29 | 7.91 |
0.15 | 0.34 | 9.13 | 0.25 | 8.93 |
0.44 | 0.40 | 18.83 | 0.32 | 15.98 |
1.78 | 0.39 | 29.25 | 0.36 | 27.0 |
a Values are based on the experiment shown in Fig 5D.