Fig 4. Triticum timopheevi private and shared alleles on chromosome 2B.

(A) F_ST plotted against r² with the KASP Sr36 assay for all SNPs on chromosome 2B. Two clusters of SNPs were manually differentiated—those with putative Triticum timopheevi private alleles for which F_ST and r² values show a general linear relationship (blue), and those with putative Triticum timopheevi/Triticum aestivum shared alleles for which F_ST shows no relationship with r² (red). Horizontal dashed line represents the 99^th percentile of genome-wide F_ST values, excluding SNPs on chromosomes 2A, 2B, and 2D. (B) Density plots of both classes of SNPs across chromosome 2B.