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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Adv Healthc Mater. 2018 Nov 22;8(4):e1801091. doi: 10.1002/adhm.201801091

Figure 1. DC-MVs directly promote T cell activation and proliferation in vitro.

Figure 1.

A. Schematic demonstrating preparation of DC-MVs. B. Western Blot analysis of MPLA-activated DCs and activation marker-enriched membrane fraction. C-D. Proliferation and activation of OT-I T cells are shown after seeding T cells at 50,000 or 10,000 cells per well in the presence of SIINFEKL alone or SIINFEKL mixed in with DC-MVs. Mean ± SD are shown. Statistical analysis was performed using two-way ANOVA comparison with Tukey's multiple comparison test comparing (MPLA)DC-MV-SIINFEKL to either DC-MV-SIINFKEL (#p < 0.05, ####p < 0.0001) or SIINFEKL alone (**p < 0.01, ****p < 0.0001).