(A) FFPE samples of three different tissue types from human, mouse, and rat were used as input into the TempO-Seq FFPE assay. Replicates were obtained by scraping adjacent areas from three different serial sections. R2 values were calculated by comparing gene expression of one section to the average of the remaining two. (B) Principal component analysis (PCA) of different tissue types of human (colon, pancreas, and prostate), mouse (breast, lung, and muscle), and rat (brain, kidney, and liver) was conducted. The first two principal components account for the majority of variance in the samples and clearly distinguish the different tissue types.