Fig 7. Gene expression correlation in biological replicates of rat FFPE tissues fixed for variable amounts of time.

(A) Rat liver tissue was harvested and fixed in 10% neutral buffered formalin for 24, 96, 192, and 384 hours. Samples were then moved into 70% ethanol and processed for FFPE and used as input into the TempO-Seq FFPE rat whole transcriptome assay. Replicates were obtained by scraping adjacent areas from three different serial sections. R² values were calculated by comparing one section to the average of the remaining two. (B) Comparison between 24-hour fixation and 96 or 384 hour fixation. R² values were calculated between averages of three biological replicates for each condition.