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. 2019 Feb 6;8:e43482. doi: 10.7554/eLife.43482

Figure 1. CX columnar neurons are generated by type II neuroblast lineages.

(A) CX columnar neurons innervating the PB originate specifically from each of four bilateral type II neuroblast lineages (DM1-DM4), which include all four neuronal subtypes shown in panel D. DM1 lineage neurons innervate the most medial PB glomeruli, and DM4 lineage neurons innervate the most lateral PB glomeruli. Adult brain right hemisphere shown. (B) Type II neuroblasts divide every 1.6 hr to generate ~60 INPs; each INP progeny divides every 2–3 hr to produce 10–12 neurons (Homem et al., 2013). Both neuroblasts and INPs express temporal transcription factors that subdivide their lineages into distinct molecular windows. Finer subdivisions exist but are not shown for clarity. (C) PF-R, E-PG, P-EN, and P-FN columnar neuron subtypes; each has a proposed function in navigation (Stone et al., 2017; ) and a distinct pattern of connectivity. PB, protocerebral bridge; FB, fan-shaped body; ROB, round body; EB, ellipsoid body; NO, noduli. (D) Adult CX columnar neurons derived from INPs labeled with adult LexA lines specific for each subtype; see Figure 1—figure supplement 1A for genetic details. ROB, red arrows; Gall, yellow arrows; Noduli, blue arrows. Scale bars, 40µm. Genotypes: PF-R, UAS-FLP; R9D11-Gal4, R37G12-lexA; lexAop(FRT.stop)mCD8::GFP; E-PG, UAS-FLP; R9D11-Gal4, R60D05-lexA; lexAop(FRT.stop)mCD8::GFP; P-EN, UAS-FLP; R9D11-Gal4, R12D09-lexA; lexAop(FRT.stop)mCD8::GFP; P-FN, UAS-FLP; R9D11-Gal4, R16D01-lexA; lexAop(FRT.stop)mCD8::GFP.

Figure 1.

Figure 1—figure supplement 1. Intersectional genetic birthdating schemes.

Figure 1—figure supplement 1.

(A) Identifying columnar neuron subtypes derived from all INPs. R9D11-Gal4 drives expression of FLP which excises an FRT-stop in all INPs and their progeny. This allows columnar neuron specific LexA lines to drive GFP expression if the neurons derive from INPs. (B) Identifying the time during the neuroblast lineage that produces each columnar neuron subtype. A pulse of 29°C disables ts.Gal80 to allow R9D11-Gal4 to excise FRT.stop in the INPs present during the heat pulse. This allows columnar neuron specific LexA lines to drive GFP expression if the neurons derive from INPs born from the type II neuroblast at the time of heat pulse. (C) Identifying columnar neuron subtypes derived from young or old INPs. OK107-Gal4 drives expression of FLP which excises an FRT-stop only in old INPs and their progeny. This allows columnar neuron specific LexA lines to drive GFP expression if the neurons derive from old INPs. Lack of expression shows the neurons are derived from young INPs. (D) Identifying the time during the neuroblast lineage that produces each old INP-derived columnar neuron subtype. A pulse of 29°C disables ts.Gal80 to allow OK107-Gal4 to excise the FRT.stop in the INPs present during the heat pulse. This allows columnar neuron specific LexA lines to drive GFP expression if the neurons derive from INPs present at the time of heat pulse.