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. 2019 Feb 5;8:e43230. doi: 10.7554/eLife.43230

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© 2019, Kol et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Author response image 1. — (B) UV-A-irradiated or dark-adapted clickSph and caSph-1 were incubated with lysates of yeast cells lacking ceramide synthases (-) or expressing human ceramide synthase CerS5 (+). Metabolic conversion to ceramide was monitored by TLC analysis of lipid extracts click-reacted with Alexa-647-azide. Note that metabolic conversion of caSph-1, unlike that of clickSph, is light sensitive.