FIG 5.

Neutrophil blockade reduces the pulmonary pathology of M. tuberculosis HN878-infected TLR2KO mice to WT level. WT and TLR2KO mice were aerosol infected with ∼100 CFU of M. tuberculosis HN878, and at days 17, 19, 21, and 24 following infection, mice were injected intraperitoneally with either anti-mouse Ly6G antibody or an isotype control. Mice were euthanized on day 27, and lungs from WT mice receiving control isotype antibody, TLR2KO mice receiving control isotype antibody, and TLR2KO mice receiving anti-Ly6G antibody, as indicated, were evaluated for neutrophil recruitment (A) and total cellular number (B). The percent lung area involved in inflammation was calculated for all three groups of mice using the ImageProJ software from Nikon (C). Scanned images of lung tissue were obtained by using Act-1 software from Nikon (D). Data are represented as means ± standard errors of the means. Statistical significance was calculated using a one-way ANOVA with Bonferroni’s correction. ***, P < 0.001; **, P < 0.01; *, P < 0.05.