FIG 5.

Immunogenicity of CS6 subunit-based monomers and dimers. Groups of 10 BALB/c mice were immunized three times, 2 weeks apart, by the i.d. route with 25 μg of each protein (CS6 or monomers or dimers of CS6 subunits CssA and/or CssB) with or without LTR192G as an adjuvant. Data are shown as means and SD of the log₁₀-transformed titers for each group, on day 42, 2 weeks after the last immunization. Baseline levels, before the first immunization, were below the limit of detection (LOD) for the assays for all mice and are not shown in the graph. The dotted horizontal line indicates the LOD (1:50 or 10^1.7). (A) Serum anti-CS6 IgG Ab titers; (B) serum anti-CssB IgG Ab titers; (C) serum anti-CssA IgG Ab titers; (D) serum anti-CS6 IgA Ab titers. Black columns, protein plus LTR192G; white columns, protein alone. *, P < 0.05.