FIG 6.

Immunogenicity of CS6 subunit-based trimers. Groups of 10 BALB/c mice were immunized three times, 2 weeks apart, by the i.d. route with 25 μg of each protein (CS6 or dimers or trimers of CS6 subunits CssA and CssB) plus LTR192G or PBS. Data are shown as means and SD of the log₁₀-transformed titers for each group on day 42. Baseline levels, before the first immunization, were below the LOD for the assays for all mice and are not shown. The dotted horizontal line indicates the LOD for the assay (1:50 or 10^1.7). (A) Serum anti-CS6 IgG Ab titers; (B) serum anti-CssB IgG Ab titers; (C) serum anti-CssA IgG Ab titers; (D) serum anti-CS6 IgA Ab titers. *, P < 0.05.