FIG 7.
Immunogenicity of refined CS6 subunit-based dimeric proteins. Groups of eight BALB/c mice were immunized three times, 2 weeks apart, by the i.d. route with 10 μg of each protein (CS6 or dimers of CS6 subunits CssA and/or CssB) plus LTR192G or PBS. Assays were performed with day 42 sera. The dotted horizontal line indicates the LOD for the assay (1:50 or 101.7). (A) Serum anti-CS6 IgG Ab titers. Data are shown as means and SD of the log10-transformed titers for each group. (B) Serum anti-CssB IgG Ab titers. Groups were assayed in pools. (C) Serum anti-CssA IgA Ab titers. Groups were assayed in pools. *, P < 0.05.