TABLE 1.

Physicochemical properties of CS6 subunit fusions

Protein	Theoretical mol wta (kDa)	Purityb (%)	Yieldc (mg/g)	Tm (°C)	HPLC-SEC
					Peak retention time(s) (min)	Mol wt (kDa)d	Oligomeric state
CssA fusions
dscACssA	18.5	95	1.3	55	10.4	20.9	Monomeric
dscACssAA	34.0	95	1.3	49.9, 75.3	9.3	34.2	Monomeric
dscBCssAB	35.0	98	8.9	58.8, 74.5	7.5, 8.0, 9.2	45.7, 94.6	Multimeric
dscACssABA	50.4	95	2.1	54.3, 75	8.5	59.2	Monomeric
dscBCssABB	51.3	94	4.0	59.5, 74	7.2, 7.5, 8.5	67.4, 132	Multimeric
Refined CssAB fusions
dscACssAB	34.8	97	4.9	70	9.2	35.5	Monomeric
ntd_dscBCssAB	33.3	99	3.8	59, 74.6	9.3	34.1	Monomeric
ntd_dscACssAB	33.2	97	3.7	69.7, 76.1	9.4	33.9	Monomeric
CssB fusions
dscBCssB	19.4	91	3.1	60.1	10.4	22.5	Monomeric
dscBCssBB	35.8	100	2.7	58, 92.6	8.1, 9.2	37.5, 94	Multimeric
dscACssBA	34.8	91	4.3	69.2, 74.7	7.7, 8.2, 9.5	39.8, 93	Multimeric
dscACssBAA	50.4	90	1.0	71.8, 77.6	7.1, 8.0	141	Multimeric
dscBCssBAB	51.3	89	1.2	59.7, 75.5	7.6, 8.0, 8.6	63.1	Multimeric
dscACssBBA	51.2	91	0.5	59, 71.4, 76	7.2, 7.9, 8.9	65.4, 122.7	Multimeric
Refined CssBA fusions
dscBCssBA	35.0	97	8.2	74.1	9.4	37.3	Monomeric
ntd_dscACssBA	33.2	97	0.5	76.1	9.4	33.7	Monomeric
ntd_dscBCssBA	33.3	98	3.0	74.7	9.3	33.9	Monomeric

^aThe theoretical molecular weight of each protein was determined from the primary amino acid sequence.

^bProtein purity was determined by densitometric measurements of the protein separated on an SDS-PAGE gel.

^cMilligrams of protein obtained per gram of wet bacterial cell paste lysed.

^dThe experimental molecular weight was determined using the refractive index of molecules eluting from the HPLC-SEC column.