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. 2019 Feb 21;87(3):e00669-18. doi: 10.1128/IAI.00669-18

FIG 5.

FIG 5

VopA inhibits JNK/Bsk phosphorylation resulting from constitutive Eiger or dTAK expression. (A) Adult Drosophila eye phenotypes resulting from constitutive Eiger expression or from constitutive coexpression of Eiger and VopA, with the genotypes indicated (top), and immunostain analysis of larval eye disks from the indicated phenotypes using antibodies against phosphorylated JNK (bottom). (B) Phenotypes of adult Drosophila eyes resulting from constitutive expression of dTAK-1 and from constitutive coexpression of dTAK-1 and VopA or mVopA, with the genotypes indicated. (C) Phenotypes of adult Drosophila eyes resulting from constitutive expression of a constitutively active form of Hemipterous [hep(act)] with VopA, from constitutive coexpression of Hemipterous (hep) and Basket (bsk), and from constitutive coexpression of Hemipterous, Basket, and VopA. (D) TUNEL analysis for the detection of apoptotic cells in the posterior midgut of flies of the indicated phenotypes. (E) Numeration of TUNEL-positive cells in panel D. ****, P < 0.0001 (n = 5). For panels A to C, representative images were observed in all eclosed adult flies of the indicated phenotype, indicating that the inhibitory effect of VopA on Eiger-induced JNK signaling in the eye occurred with 100% penetrance.