FIG 7.

Vaccination with MAV preparations increases the survival of mice and alters the inflammatory response after S. pneumoniae TIGR4 pneumonia challenge. (A) Percent survival of mice over 6 days after challenge by intranasal inoculation with 1 × 10⁷ CFU of the S. pneumoniae TIGR4 strain of mice that had been vaccinated three times (days 1, 10, and 22) intraperitoneally with 75 μg of MAV^IPS014 or a negative-control buffer (n = 15 per group). Significance was calculated using the log-rank (Mantel-Cox) test. (B to D) Numbers of CFU in target organs (B) and inflammatory cell populations in BALF (C) and lung (D) 24 h after challenge with 1 × 10⁷ CFU of the S. pneumoniae TIGR4 strain in MAV^IPS014-vaccinated and control mice. Inflammatory cell data are shown as a percentage of the total cells recovered from the lungs of MAV- and buffer-vaccinated mice; CFU data show the number of CFU in lung, blood, or BALF recovered 24 h after challenge, with each symbol representing data from a single mouse and horizontal bars representing median values. (E to H) Lung homogenate cytokine levels 24 h after challenge with 1 × 10⁷ CFU of the S. pneumoniae TIGR4 strain in MAV^IPS014-vaccinated and control mice. For panels B to H, statistical significances were calculated using a Mann-Whitney t test. Significance abbreviations: *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.