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Western blot analysis of pyrin S205 dephosphorylation in BMDMs. LPS-primed BMDMs were left uninfected or infected with the indicated Y. pseudotuberculosis 32777 ΔyopM strain for 90 or 180 min. After infection, lysates were processed with denaturing RIPA lysis buffer, collected, and subjected to Western blotting using antibodies to pSer205 of pyrin, total pyrin, or β-actin.
