Figure 1.

Large Cell Size Impairs Cell Proliferation

(A) Left: Logarithmically growing cdc28-13 cells were shifted to 37°C under the indicated growth conditions (CHX = cycloheximide) and volume was determined using a coulter counter. Right: Representative images of a cell before and 6 h after shift to 37°C grown in 2% glucose.

(B) 10-fold serial dilutions of cdc28-13 cells arrested at 37°C as indicated were plated and grown at 25°C. (C–H) cdc28-13 cells expressing Whi5-tdTomato and Spc42-GFP (C–E, G, H) or CLN2pr-GFP (F) were arrested at 37°C under the indicated conditions. Cells were shifted to medium containing 2% glucose lacking drugs at 25°C and cell-cycle progression was monitored. Asterisks indicate p < 0.05 (Mann-Whitney U test).

See also Figures S1, S2, S3.