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. 2019 Feb 21;176(5):1083–1097.e18. doi: 10.1016/j.cell.2019.01.018

Figure 5.

Figure 5

Low DNA:cytoplasm ratio causes large cell phenotypes

(A–C) Haploid (1n) and diploid (2n) cdc28-13 cells expressing Whi5-tdTomato were arrested for different times in G1 to reach an equal cell size (arrest times: 1n: 3 h 30 min, 6 h 15 min; 2n: 2 h 15, 3 h 30 min). Cells were shifted to 25°C and imaged.

(D and E) Haploid and diploid cdc28-13 cells expressing GAL1pr-GFP were arrested in raffinose for different amounts of time for cells to reach the same size (arrest times: 1n: 1 h, 4 h, 6 h; 2n: 1 h 30, 3 h, 5 h). GAL1pr-GFP was induced by addition of 1% galactose and GFP expression analyzed by FACS 3 h after galactose addition.

(F and G) haploid (MATa) and diploid (MATa/alphaΔ) cdc28-13 cells were arrested for different amounts of time for cells to reach the same size (arrest times: 1n: 1 h 20 min, 3 h 45 min, 6 h 15 min; 2n: 1 h 20 min, 2 h 20 min, 3 h 45 min, 6 h 15 min) and exposed to alpha factor for 5 min to analyze Fus3 phosphorylation.

See also Figure S7.