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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Curr Protoc Cell Biol. 2018 Sep 28;82(1):e65. doi: 10.1002/cpcb.65

Table 2.

Pre-coating cells with specific antibody

Antibody Tube
Blank Single color control
Sample
Lin CD49f EpCAM
Anti CD31 - 1 μL - - 1 μL for 106 cells
Anti CD235a - 1 μL - - 1 μL for 106 cells
Anti CD45 - 1 μL - - 1 μL for 106 cells
Brilliant Violet 605Streptavidin - 0.5 μL 0.5 μL
Anti CD49f-APC - - 3 μL - 3 μL for 106 cells
Anti EpCAM-FITC - - - 3 μL 110 μL for 106 cells