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. Author manuscript; available in PMC: 2020 Feb 21.
Published in final edited form as: Mol Cell. 2019 Jan 29;73(4):727–737.e3. doi: 10.1016/j.molcel.2018.12.015

Figure 6. Detection of Type V-C Cas1 Tetramer by Native Mass Spectrometry.

Figure 6.

(A) NanoESI mass spectra of cross-linked samples of apo Cas1. Measured molecular masses and corresponding cartoons of each species are listed on the top right corner. Ions of different species are labeled with different colors.

(B) NanoESI mass spectra of cross-linked samples of Cas1 after complexing with pseudo-full-site substrate. See also Figure S6.

(C) Model for type V-C mini-integrase compared to canonical Cas1-Cas2 integrase.

See Table S2 for nucleotide sequences.