Skip to main content
. Author manuscript; available in PMC: 2020 Feb 21.
Published in final edited form as: Cell Chem Biol. 2018 Dec 6;26(2):213–222.e6. doi: 10.1016/j.chembiol.2018.10.019

Figure 1. The spectral shift of TSQ used for islet visualization is reversible.

Figure 1.

(A) Chemical structures of 6-methoxy-8-p-toluenesulfonamido-quinoline (TSQ, left) and N’,N’,N,N-Tetrakis(2-pyridylmethyl)-ethylenediamine (TPEN, right). (B) Representative images of TSQ-treated (10 μM) islets in the absence (left) and presence (right) of the Zn2+ chelator TPEN (1 mM). Staining was conducted in 6-well format (n=3) with 50 islets per well, and at least 5 images per condition were taken per well. Yellow arrows indicate unstained exocrine tissue. (C) Fluorescence quantification by FACS of R7T1 β-cells (left) and HEK 293T cells (right) following TPEN, TSQ, and TSQ/TPEN (green) exposure (n=2–4). (D) Zinc quantification by ICP-OES of HEK 293T, exocrine pancreas, R7T1 β-cells (R7T1), and pancreatic mouse islets (n=3–4). Data are represented as mean ± S.D. Comparisons with p > 0.05 are indicated by NS (not significant); 0.01<p<0.05 by *; 0.001<p<0.01 by **; 0.0001<p<0.001 by ***; and p<0.0001 by ****.

See also Figure S1.