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. Author manuscript; available in PMC: 2020 Feb 21.
Published in final edited form as: Cell Chem Biol. 2018 Dec 6;26(2):213–222.e6. doi: 10.1016/j.chembiol.2018.10.019

Figure 2. TSQ accumulates in a Zn2+- and cell type-dependent manner.

Figure 2.

(A) TSQ quantification (LC-MS/MS) from TSQ/CC-401-treated R7T1 β-cells (R7T1) without (blue) or with TPEN (red) co-treatment (n=20–32). (B) CC-401 quantification (LC-MS/MS) for the experiment in (A) monitored simultaneously with TSQ with vehicle (blue) or TPEN (red) co-treatment (n=20–32). (C) TSQ/CC-401 ratio for R7T1 with vehicle (blue) or TPEN (red) co-treatment (n=20–32). (D) TSQ quantification (LC-MS/MS) in primary mouse islet and exocrine tissues following TSQ/CC-401 treatment with vehicle (blue) or TPEN (red) co-treatment (n=24–29). (E) CC-401 quantification (LC-MS/MS) monitored simultaneously with TSQ in (D) (n=24–29). (F) TSQ/CC-401 ratio for mouse islet and exocrine tissues (D and E) (n=24–29). (G) TSQ quantification (LC-MS/MS) of TSQ/CC-401-treated human islets, co-treated with vehicle (blue), [TPEN]=100nM (red), or [TPEN]=10nM (green) (n=8–12). (H) CC-401 quantification (LC-MS/MS) determined simultaneously with TSQ in (G) following vehicle (blue), [TPEN]=100 nM (red), or [TPEN]=10 nM (green) co-treatment; (n=8–12). (I) TSQ/CC-401 ratio for human islets, co-treated with vehicle (blue), [TPEN]=100 nM (red), or [TPEN]=10 nM (green) (n=8–12). Data are represented as mean ± S.D. Comparisons with p > 0.05 are indicated by NS (not significant); 0.01<p<0.05 by *; 0.001<p<0.01 by **; 0.0001<p<0.001 by ***; and p<0.0001 by ****.

See also Figure S2.