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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Curr Protoc Cell Biol. 2018 Sep 28;82(1):e64. doi: 10.1002/cpcb.64

Table 1.

Probes information and conditions

Probe Probe sequence Target Hybridization temperature (°C) Probe concentration Conjugation Additional immune detection
DNA-LTR (PNA) CAT ATA AGC AGC TGC TTT TTG CCT GTA CTG GGT HIV-LTR 42° 10μM 5′-Biotin or direct conjugation to any color Yes
DNA-Nef (PNA) GCA GCT TCC TCA TTG ATG G HIV-Nef 42° 10μM 5′-Biotin or direct conjugation to any color Yes
DNA-Alu (PNA) GCC TCC CAA AGT GCT GGG ATT ACA G Alu repeats 42° 10μM 5′-Biotin or direct conjugation to any color Yes
RNA-Gag- Pol (BNA) Advanced Cell Diagnotics Catalog # 317691 Gag- Pol mRNA 40° 1μM 5′-Biotin or direct conjugation to any color Yes
RNA-Stellaris probes Biosearch technologies Nef mRNA 40° See commercial information See commercial information Limited, single molecule detection, but analysis by confocal is limited