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. Author manuscript; available in PMC: 2020 Feb 21.
Published in final edited form as: Cell Chem Biol. 2018 Dec 20;26(2):269–277.e5. doi: 10.1016/j.chembiol.2018.10.025

Figure 3.

Figure 3.

Crystal structure of hADAR2-D E488Y bound to reduced abasic containing RNA substrate. (A) Duplex RNA for crystallization. Gli1 duplex has sequence surrounding the human Gli1-mRNA editing site. Adenosine analog 8-azanebularine (N) is across from reduced abasic site (rAb). N allows for trapping of protein-RNA complex in base flipped conformation. (B) Intercalation of Y488 from the minor groove side of RNA duplex in base-flipped conformation. Potential hydrogen bonding at N4 amine of C10’. (C) Orphan base, reduced abasic site (rAb), maintains RNA backbone found in other ADAR-RNA structures. Side chain of R510 ion-pairs with the 3’-phosphodiester of orphaned base, rAb11’. See also Table S1 and Figure S2.