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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: J Leukoc Biol. 2018 Dec 11;105(3):539–549. doi: 10.1002/JLB.4A0518-191R

FIGURE 3. Neonatal CD31+ CTLs produce less IFN-γ at the site of infection, as compared to circulating neonatal CTLs.

FIGURE 3

Day 9 post-influenza virus infection, neonatal CD8+ T cells were labelled via intravenous anti-CD8a monoclonal antibody labelling via retro-orbital injection to identify those cells within the lung tissue. Cells were stimulated ex vivo following intravenous labelling with immunodominant peptide NP(366–374). (A) Representative flow plots depicting IV versus Lung labeled CD8a+ T cells and gating strategy. (B) Frequency of NP(366–374) tetramer positive effector CTLs within the circulation. (C) Number of CD31+ and CD31− NP(366–374) tetramer positive effector CTLs within the lung. (D) Ratio of the frequency of NP(366–374) CD31+ or CD31− effector CTLs to the frequency of the total NP(366–374) positive effector CTLs within the circulation and within the lung (E). (F) Frequency of IFN-γ+ effector CTLs within the circulation. (G) Absolute number of IFN-γ+ effector CTLs within the lung tissue. (n = 5–8 animals per group, 2 independent experiments). Paired T test (nonparametric statistics were used to compare groups by CD31 expression (*P < 0.05, **P < 0.005, ***P < 0.0005, and ****P < 0.0001)