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Mutational and substrate specificity studies of the Ebony CN domain. (Left) Condensation activity observed for the wild-type CN domain and mutants using β-alanyl-CoA 1 and dopamine (triplicate experiments). (Right) β-aminoacyl-CoA analogs tested with the CN domain (2–7), indicating their viability as substrates for the condensation reaction with dopamine (glycyl-CoA and α-aminoacyl-CoAs are not accepted by CN).
