Fig. 5.
IL-27–stimulated CD39high Tregs mediate suppression via enhanced enzymatic activation. Foxp3+ cells from IL-27–stimulated and unstimulated Tregs (A, nTregs; B, iTregs) were incubated with exogenous ATP. The remaining ATP was measured in the supernatants, and percent ATP hydrolysis against control is shown. (C) The effect of iTregs or IL-27-iTregs on cytokine-producing CD8+ T cells. (D and E) The effect of CD39 blockade by ARL67156 on (D) ATP hydrolysis and (E) IL-2 production in CD8+ T cells. The representative graphs and plots are shown. The graphs show means ± SEM (*P < 0.05, **P < 0.01, and ***P < 0.001). Data are representative of two independent experiments.