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. 2019 Feb 4;116(8):3062–3071. doi: 10.1073/pnas.1815966116

Fig. 3.

Fig. 3.

Genetic anticipation in the reduction of RLS of strong diploid mutators. The pol3-01/pol3-01 msh6Δ/msh6Δ zygotes were manually isolated from a cross of pol3-01 msh6Δ haploid cells that had undergone three or fewer cell divisions after tetrad dissection. RLSs of the first diploid daughter cells from these zygotes (fresh isolates, blue; median RLS = 22, n = 21) were compared concurrently with previously passaged pol3-01/pol3-01 msh6Δ/msh6Δ cells (control, red; median RLS = 6, n = 60, P < 0.001) and the POL3/pol3-01 MSH6/msh6Δ parent strain (parent strain, black; median RLS = 27, n = 60, P = 0.075). Daughter cells dissected from the fresh isolates were grown into colonies. Six colonies were subcloned twice to obtain two additional RLS cohorts (d3, gray; d4, purple) (combined median RLS = 8, n = 240, P < 0.001). Comparisons used the Wilcoxon rank sum test with a Bonferroni multiple testing correction. Genotype grid: 3-01, pol3-01; m6, msh6; filled triangles, heterozygous; filled squares, homozygous.