. 2019 Feb 4;116(8):3091–3099. doi: 10.1073/pnas.1817621116

Table 1.

Predicted regulators of C₄ enzyme genes

Target C₄ enzyme gene^*	TF gene name	TF gene ID	\| R_i \|^†	Available^‡/purchased	Purified^§/EMSA
BS: ZmNADP-ME	ZmGATA12	Zm00001d037605	0.76	✓/✓	✓/✓
BS: ZmNADP-ME	ZmbHLH43	Zm00001d033267	0.98	✓/✓	✓/✓
BS: ZmNADP-ME	ZmERF	Zm00001d052229	0.83	✓/✓
BS: ZmNADP-ME	ZmNAC	Zm00001d050893	0.76	✓/
BS: ZmPCK	ZmMYB48	Zm00001d041576	1.00	✓/✓	✓/✓
BS: ZmPCK	ZmMYB88	Zm00001d048623	1.00	✓/✓	✓/✓
BS: ZmPCK	ZmMYB56	Zm00001d030678	1.00	✓/✓	✓/✓
BS: ZmPCK	ZmbHLH118	Zm00001d038357	0.72	✓/✓	✓/✓
BS: ZmRBCS2	ZmMYBr17	Zm00001d044409	0.91	✓/✓	✓/✓
M: ZmCA	ZmABI33	Zm00001d011639	0.97
M: ZmCA	ZmRAV	Zm00001d043782	0.91
M: ZmPEPC	ZmRAV	Zm00001d043782	0.91

BS: preferred expression in bundle sheath cells. M: preferred expression in mesophyll cells.

^{^†}

Degree of cell-type preference of gene i is defined as | R_i | = |m_i − b_i|/max(m_i, b_i), where m_i and b_i represent the RPKM value of gene i in the M and BS RNA samples, respectively (20).

^{^‡}

Available in the Maize TFome Collection; the ZmNAC clone was not ordered because this candidate TF was predicted after we sent out the purchase order of TF clones.

^{^§}

Expression of the ZmERF clone failed.