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. 2019 Feb 4;116(8):2935–2944. doi: 10.1073/pnas.1808696116

Fig. 3.

Fig. 3.

RRM3 recognizes various motifs in solution and binds multiple registers. (A) Close-up view of the 1H-15N HSQC spectrum for key residues showing different chemical shift perturbations for AUUUU or AUUAU compared with UUUUU [RRM3:RNA ratios: 1:0 (blue), 1:0.25 (red), 1:0.5 (yellow), 1:1 (green), 1:1.5 (cyan), and 1:2.5 (magenta)]. (B) Residues with chemical shift differences larger for AUUUU and AUUAU than for UUUUU are represented as sticks and highlighted in green on chain A. (C) Comparison of H5–H6 correlations in 2D TOCSY spectra at 750 MHz of RRM3 in complex with various 6-mer RNAs (blue, 5′-UUUUUU-3′; orange, 5′-AUUAUU-3′; green, 5′-UAUUUA-3′). (D) Close-up view of the 1H-15N HSQC spectrum for key residues showing a different exchange regime for AUUAUU compared with UUUUU or UAUUUA. RRM3:RNA ratios: 1:0 (blue), 1:0.25 (red), 1:0.5 (yellow), 1:1 (green), 1:1.5 (cyan), and 1:2.5 (magenta).