Fig. 5.
Dimerization and RNA binding of RRM3 directly affect each other. (A) Comparison of ITC profiles of RRM3 or W261E titrated with UUUUUU or c-fos. Errors indicate the SD of at least two measurements. (B) Ka in M−1 (thick line, Right axis) and N value (thin line, Left axis) measured by ITC for RRM3 (red) and W261E (black). Errors indicate the SD of two or three measurements. (C) Model of RNA binding-induced dimerization. Binding to long RNAs, comprising two motifs separated by a long linker, induces dimerization.